Estabelecimento in vitro de explantes de Eugenia Involucrata DC

Abstract

The Eugenia involucrata DC., Popularly known as cherry-the-Rio-Grande belongs to the Myrtaceae family, is native mainly from Southern Brazil, occurring from Rio Grande do Sul to Minas Gerais. Use in folk medicine is known to possess digestive, antidiarrheal and antirreumática action. The species is widely used for its excellent training pantry and reclamation. Micropropagation and offer the possibility of propagation of selected trees, enables clonal cleaning, obtaining virus-free plants by means of stem and root tips (primary meristems), overcoming problems of pathogen contamination. The aim of this study was to establish a protocol for micropropagation E. involucrata through in vitro germination of their seeds. Ripe fruit with no sign of pest attack were collected directly from two parent trees were pulped manually with the aid of a knife, the seeds were disinfected with two treatments, the first with 70% alcohol solutions (one minute) and sodium hypochlorite 1.25% (15 minutes) and the second with the two previous solutions followed by one minute of immersion ultrasound, 200 seeds were inoculated in agar-water for germination, after germination, the shoots were excised and inoculated in MS containing four different concentrations of BAP (0.02%, 0.04%, 0.1%, 0.16%) for the development of shoots. Later was made a rooting medium for containing 00.1% of BAP and 0.02% IBA, where 32 explants were inoculated. Were also inoculated roots for verification of germination on MS medium containing BAP AIB 0.00025% and 0.005%. It was concluded that alcohol along with hypochlorite has been more successful in disinfestation. It was also found that the nodal segment meristems form shoots when used in greater numbers than 0.00025% and 0.02% of IBA BAP. The rooting of the explants was visualized under the concentration tested. There is no evidence of sprouting roots.