Efeito protetor do suco integral de amora-preta (Rubus spp.) em um modelo de dano renal induzido por cisplatina em camundongos

Abstract

The chronic consumption of whole black mulberry juice (WBMJ) is related to the prevention of urinary tract diseases in humans. However, the evaluation of the effect of the subchronic intake of WBMJ in the urinary system is poorly studied mainly in non-infectious models. This work aimed to evaluate the effect of the oral administration of WBMJ in a renal damage induced by cisplatin in mice. A preliminary assessment of the levels of anthocyanins in WBMJ was performed, and the prevalence of Cyanidin 3-O-glucoside and Cyanidin 3-O-rutinoside was verified. Mice were orally administered with WBMJ (10ml/kg) for seven days. On the seventh day mice were injected with cisplatin intraperitoneal (10mg/kg). After 72 h, the animals were anesthetized and blood was collected and used for the evaluation of urea and creatinine levels. Kidneys were removed and used for the analysis of thiobarbituric acid reactive species (TBARS), ascorbic acid (AA), and non-protein thiols (NPSH) levels. Superoxide dismutase (SOD), glutathione S-transferase (GST) and catalase (CAT) activities were also assessed. The cisplatin administration caused increased urea and creatinine levels, and inhibited SOD, GST and CAT activities. The administration of WBMJ protected against the increase in urea and creatinine levels, and the inhibition of the CAT activity. However, it did not prevent the inhibition of SOD and GST activities induced by cisplatin. In conclusion, WBMJ protected the renal system of mice from the deleterious effects of the administration of cisplatin through antioxidant mechanisms. Due to the abundance of anthocyanins cyanidin 3- O-glucoside and cyanidin 3-O-rutinoside, it is possible that these compounds are involved synergistically or individually in antioxidant role of WBMJ. In a more specific form, it can be inferred that these anthocyanins could be related to the regulation of CAT in the renal tissue, because this enzyme was apparently the mechanism of nephroprotective effect of WBMJ.