Avaliação da expressão diferencial em Physcomitrium patens na busca das adaptações moleculares responsivas na arquitetura dos órgãos reprodutivos

Abstract

Infertility is linked to different functions of male gametes, one of which is caused by impaired motility due to anomalies in sperm flagella. Several genetic defects culminate in the loss of fertility when viewed from an evolutionary perspective, where sperm flagellar function is extremely conserved across all kingdoms. The model moss Physcomitrium patens has genes copies homologous to human that are related to the architecture of microtubules that enable sperm motility. The moss P. patens has been an important model system for studying issues of evolutionary and developmental biology, as well as being an excellent model for studies of cellular reprogramming, in addition to collaborating with studies of flagellated organisms from various domains of biodiversity. Although the genes involved in the process of organogenesis of the reproductive structures of mosses are still little explored, it is believed that these may be involved in the construction of the motility of the flagellum and other elements of the architecture of the tissues and organs involved in evolution. Based on this hypothesis, our objective is to identify the genes responsible for the differentiation of reproductive structures and how these can identify problems in the formation of Organs reproductive organs of mosses. To achieve the objectives, a differential gene expression analysis was carried out with the aid of data obtained in the RNA-Seq technique. The reads used were obtained from the NCBI (National Center for Biotechnology Information) database, on the Sequence Reads Archives (SRA) platform SRR19502729, SRR19502730, SRR19502731, SRR19502732, SRR19502733, SRR19502734, SRR4454535 and SRR9901085. The raw reads were filtered by size and quality (Phred-Score 28) and then analyzed for transcript counts and differential gene expression analysis (DEGs) using the Salmon tool. As a result, the genes Pp3c10_9456v3.1, Pp3c17_1640V3.1 and Pp3c17_13470V3.1 were identified as possible genes involved in the sexual differentiation between sexual organs of moss, where these genes are over-expressed when there is formation of antheridia and under-expressed when there is formation of antheridia. archegoniums. Through the analysis of gene function and ontology, it was observed that the Pp3c10_9456v3.1 gene is responsible for the determination of symmetry, morphogenesis of the anatomical structure, assembly of cellular components and development of organs in P. patens, being an ideal target for tests of gene knockout to validate its role in the differentiation of reproductive organs in this plant.