Efeito do ácido siríngico nas alterações comportamentais e oxidativas induzidas por cloreto de alumínio em drosophila melanogaster

Abstract

The chemical elemento aluminum (Al) is the most abundant metal in the Earth’s crust and its use on a large scale has been increased human exposition. In this context has been demonstrated that Al presented toxic effects at high doses, affecting mainly the CNS and it is correlated with increased incidence of neurodegenerative diseases. Although the mechanism(s) by which Al exerts toxicity are not completely elucidated, it has been suggested oxidative stress as a mainly mechanisms of Al toxicity which also is associated with neurodegenerative process. Moreover, treatments to Al intoxication are still scarce. However, it has been beneficial effects of antioxidants from natural origins as way of treatment and prevention of oxidative damages caused by toxic agents among which we highlight the Al. Syringic acid is a phenolic acid found in cereals and has antioxidante, anti-inflammatory and hepatoprotective effects described but little explored. Thus, the aim of this study was to evaluate the effect of syringic treatment, a phenolic compound with antioxidante action, against toxic effects from the exposition to Al in a fruit fly D. melanogaster experimental model. Ours results shows that Al exposition (5mM) significantly increased (P<0,05) mortality and reduced locomotor activity (p<0,05) of flies. Treatment also changed oxidative stress markers, increased TBARS levels (p<0,05), reduced catalase activity (p<0,05) in heads and bodies and reduced (p<0,05) NPSH groups levels in fruit flies head. Besides, reduced significlantly (p<0,05) AChE activity and cellular viability (MTT) in head. On the other hand, syringic acid treatment (25 μM) significantly (p<0,05) protected mostality and locomotor déficit caused by Al. Treatment also reduced TBARS levels (p<0,05) in head and body, avoided the reduction of catalase activity (p<0,05) in head and the reduction of NPSH groups levels (p<0,05) in body, prevent the reduction of AChE activity (p<0,05) and cellular viability (p<0,05) in head’s flies. In view of this results, it is suggested syringic acid has neuroprotective effect against Al exposure effects. However, more studies are necessary to search a better understanding of responsible mechanism(s) for these effects both Al and syringic acid observed in this study.