Avaliação da atividade antioxidante e farmacológica de duas pirazolilbenzenosulfonamidas em Drosophila melanogaster

Abstract

Medicines are increasingly effective and abundant, being possible to highlight the pyrazoline derivatives, which have analgesic, anti-inflammatory and antipyretic action. Also highlighted are antioxidants, free radical inhibitors. The compounds 4- (5- hydroxy-3- (4-methoxyphenyl) -5- (trifluoromethyl) -4,5-dihydro-1H-pyrazol-1-yl) benzenesulfonamide (1a) and 4- (4-methoxyphenyl -5-trifluoromethyl-1H-pyrazol-1- yl) benzenesulfonamide (1b), are analogous to pyrazolylbenzenesulfonamide (Celecoxib), and were tested in this work, aiming the analysis of in vitro and in vivo results of compounds 1a and 1b and antioxidant and pharmacological activities in Drosophila melanogaster. In vivo and in vitro tests were performed. The in vitro tests were the scavenging of DPPH⁺ and ABTS⁺. In vivo tests were: survival, open field and negative geotaxis for flies receiving different concentrations of compounds 1a and 1b, observed after 24 and 48 hours. Compound 1a showed antioxidant activity only in the ABTS⁺ test. There was no antioxidant activity for compound 1b in the in vitro tests. Higher survival was observed for flies that received 1 μM of compound 1a. For compound 1b there was greater survival at the concentration of 10 μM. For the open field test there was better locomotor behavior after 24 hours for flies treated with 10 μM and after 48 hours for flies treated with 10 and 50 μM of compound 1a. For compound 1b the 1 μM concentration was highlighted after 24 hours and 10 μM after 48 hours.To negative geotaxis, the concentrations of 1 and 50 μM were stressed after 24 hours and 25 μM after 48 hours for compound 1a. For compound 1b, better levels were detected than the control in all cases, both after 24 hours and 48 hours. This study allowed to identify characteristics of the action of compounds 1a and 1b in Drosophila melanogaster flies. We highlight the relevance of further studies on compounds 1a and 1b.